C3H8O3
-20°C
T4 DNA Ligase is highly efficient, accurate and rapid enzyme designed for an efficient ligation of cohesive and blunt ended DNA insert into plasmid vectors in 5-15 minutes. It is based on the combination of T4 DNA Ligase with a Premium 5x Ligation Buffer. The ligation reaction mixture is used directly for bacterial transformation using conventional transformation procedures. It enables sticky end or blunt end DNA ligation in only 5 minutes at Room Temperature.
Advantages & Features:
• Recombinant: isolated from E. coli
• Highly efficient: 3,500 white colonies and 10 blue colonies expected under optimal conditions
• Fast and easy protocol: get 24 % of efficiency at 5 minutes and 75 % at 15 minutes
• Powerful: allows cloning insert amounts < 1 ng/kb
• Versatile: enables sticky end or blunt end DNA ligation
Applications:
• Routine cloning procedure: blunt and cohesive double stranded DNA cloning
• TA cloning
• Joining of adaptors with blunt or cohesive ends
• Cloning of restriction enzyme generated DNA
fragments
• Cloning of PCR products
• Joining of double-stranded oligonucleotides linkers or
adaptors to DNA
• Site-direct mutagenesis
• Amplified fragment length polymorphism
• Ligase-mediated RNA detection
• Nick repair in duplex DNA, RNA or DNA/RNA hybrids
• Self-circularization of linear DNA
0.1 lbs
Research or further manufacturing use only, not for food or drug use.
